Cell Line Development (CLD) is an integral aspect of biopharmaceutical manufacturing and involves the engineering of cell lines to produce various biologics. CLD allows researchers to specifically tailor cell cultures and identify candidates that provide high yields of molecules with the desired characteristics.
A robust and reliable cell line is crucial for large-scale manufacturing of biologics and therapeutics. Whilst traditional CLD methods such as Limiting Dilution (LD) and Fluorescence Activated Cell Sorting (FACS) have been effective - they have many challenges including high cost, poor monoclonality, and growth. For these reasons, automation is becoming more prevalent in CLD to increase throughput and accuracy for labor-intensive workflows.
The High-throughput Nanowell-based Image Verified Cloning (HT-NIC) protocol is one such method and using Sartorius’ CellCelector Flex platform and nanowell plates elevates CLD workflows by allowing the generation and isolation of clones in a single cloning round with proof of monoclonality.
In this infographic, you will learn about:
- Cell Line Development (CLD) and how it works
- Traditional methods for CLD and their pros and cons
- Automation in CLD and HT-NIC to simplify CLD workflows
- Sartorius’ CellCelector Flex platform and established HT-NIC workflow
Download the infographic now to learn how to fast-track your lab for the next generation of single-cell cloning.